5/31/2021Purpose: To investigate the vasodilating effect of Hypericum revolutum Vahl (Clusiaceae) in rats.
Methods: H. revolutum aerial parts were extracted with methanol. The total methanol extract was fractionated with chloroform to yield fraction I. The remaining aqueous solution was chromatographed on Diaion HP-20 using water, 50 % methanol, and methanol to yield three fractions (II- IV). Total methanol extract and fractions I-IV were applied to phenylephrine-pre-contracted (10 μM) rat aortic rings at doses of 1, 3, and 10 μg/mL. Subsequent decreases in aortic tension were recorded by an isometric force transducer to evaluate the vasodilation. Column chromatography was utilized to separate the active components of the bioactive fraction.